Question: (Closed) predicting Alternative splicing
0
gravatar for rajesh
4.1 years ago by
rajesh 60
India/Chandigarh/
rajesh 60 wrote:

I want to predict alternatively spliced exons in human genome. So which dataset i choose i.e RNA seq or Whole exome sequencing. Can anyone tell me.

rna-seq chip-seq alignment genome • 1.3k views
ADD COMMENTlink modified 4.1 years ago by Chris Cole760 • written 4.1 years ago by rajesh 60

Hello rajesh !

We believe that this post does not fit the main topic of this site.

OP does not respond to posts after asking questions. Question closed until OP responds.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink written 2.9 years ago by RamRS30k
3
gravatar for Satyajeet Khare
4.1 years ago by
Satyajeet Khare1.6k
Pune, India
Satyajeet Khare1.6k wrote:

RNA-Seq. Exome sequencing is sequencing of DNA and not RNA.

ADD COMMENTlink written 4.1 years ago by Satyajeet Khare1.6k
1
gravatar for Chris Cole
4.1 years ago by
Chris Cole760
Scotland
Chris Cole760 wrote:

Using RNA-seq (or exome) data is not a prediction, it is searching for alt-spliced exons. As far as I know there is no reliable method for predicting alt-splicing without RNA data.

Alt-splicing is also tissue and developmental time point dependent so which dataset you use will affect which splice sites you see.

ADD COMMENTlink written 4.1 years ago by Chris Cole760
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