Question: Why do illumina forward reads have better quality than the reverse reads?
2
gravatar for ali.shojaee.bakhtiari
2.5 years ago by
ali.shojaee.bakhtiari20 wrote:

I have noticed that when both forward and reverse reads from a sample are present, the forward reads tend to have better read qualities, why is it so? What is the deciding factor?

sequencing rna-seq • 866 views
ADD COMMENTlink written 2.5 years ago by ali.shojaee.bakhtiari20
1

I've always thought this had to do with the imaging as your clusters are a bit fuzzier by the time you get to R2. Whether this is due to additional amplification or the longer amplicons, I'm not entirely clear on.

ADD REPLYlink written 2.5 years ago by fanli.gcb660
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