homology of primers for duplex qPCR
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7.4 years ago
Yael • 0

Hi, I would like to check if there is any homology between 2 sets of primers for my TaqMan qPCR. I've checked homology of primer set for target and for endogenous control individually in the Primer-Blast using nr and RefSeq databases. Each pair set was OK individually and it will amplify only target or endogenous control.

How can I put them together and see if there is any chance that they bind to each other? And, is it any point to align them in e.g. EndNote?

Thanks. Yael

blast sequence genome • 1.1k views
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