Question: Isoform Differential Expression Analysis
0
gravatar for john
2.6 years ago by
john30
Canada
john30 wrote:

So basically I want to investigate whether there is a switch in isoform preference between condition 1 and condition 2. My idea was to conduct differential expression analysis on the data. If isoformA have decreased differential expression and isoformB have increased differential expression from condition 1 to condition 2, there is a shift in isoform preference.

Fortunately, I have rsem data so I just need a tool that does what I want which is also my issue. From doing my own investigation rsem tends to be paired with EBseq for analysis, so I looked up the manual from EBseq. Based on the manual, the output of EBseq only informs whether an isoform is differentially expressed or equivalently expressed based on your FDR. I need a tool that gives some fold change.

Tools like DESeq2 will give me the results in the form I want but according to various posts, using isoform counts will violate the underlying assumptions of these tool.

Is there any tool that is made for isoform data and produces output that i want or is it acceptable to adapt DESeq2 to my purposes?

rna-seq rsem deseq2 ebseq • 2.2k views
ADD COMMENTlink modified 2.2 years ago by kristoffer.vittingseerup2.2k • written 2.6 years ago by john30
1
gravatar for EVR
2.5 years ago by
EVR540
Earth
EVR540 wrote:

Take a look at DEXseq. SInce you have RSEM data it is worth to take a look at the enter link description here

ADD COMMENTlink written 2.5 years ago by EVR540
1
gravatar for kristoffer.vittingseerup
2.2 years ago by
European Union
kristoffer.vittingseerup2.2k wrote:

I would recommend IsoformSwitchAnalyzeR ( http://bioconductor.org/packages/IsoformSwitchAnalyzeR/ ) which directly parses RSEM (count) output (also support cufflinks/cuffdiff, Kallisto, Salmon). IsoformSwitchAnalyzeR enables statistical identification of isoform switches with predicted functional consequences where the consequences can be chosen from a long list but includes gain/loss of protein domains, signal peptides changes in NMD sensitivity etc.

For other inspiration of other post-analysis of isoform switches take a look at http://mcr.aacrjournals.org/content/early/2017/06/02/1541-7786.MCR-16-0459

Hope this helps

Kristoffer

ADD COMMENTlink written 2.2 years ago by kristoffer.vittingseerup2.2k
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