Transcript consensus sequence for variant calling analysis
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Entering edit mode
7.2 years ago
bharata1803 ▴ 560

Hello,

I want to ask a bit general question about variant calling workflow. In bcftools, we can generate consensus sequence for te variation we find. I have tried it with human genome reference and it can generate consensus file in fasta format. My question is, is it possible to generate consensus sequence from transcript reference (cDNA human reference)? What I imagine is we can get fasta file for transcript sequence with variation in it. Thank you.

sequencing exome-seq • 1.5k views
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