Question: UCSF chimera Minimization (after replacing WT amino acid with Mutant)
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gravatar for sv91707
4.0 years ago by
sv9170710
Tampere
sv9170710 wrote:

Hi,

I'm currently visualizing a mutation in UCSF chimera. The mutation has been confirmed, I'm looking at the moment how it may destabilize protein.

I can easily see clashes with nearby atoms in UCSF chimera when I replace WT AA with MUT AA. My question is what is the point of minimization ?

I tried it and it took till the end of the default steps (100) to remove all clashes, and number of contacts to zero. Does number of steps to minimize structure has any significance ? Also, max rotamer probability is .12,with next .09 and .08 and so on.

minimization ucsf chimera • 1.7k views
ADD COMMENTlink written 4.0 years ago by sv9170710
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