Meaning of Per sequence quality scores in FastQC

Question

[khatami.s.83]

Hi all,

Can anybody help me to understand the meaning of Per sequence quality scores in FastQC analysis? . The y-axis, what is mean? anybody can explain the concept to me , that will be great help?

Thanks,

Somayeh

Answer 1

[mirzaei86.vahid]

Dear Somayeh

Per sequence quality shows overall read count for each quality scores, for example if you got highest peak for "30" it means Q30 have more read number than other quality scores. X axes = Quality scores and Y axes= Number of reads.

To get me more information please take a brief look at following links:

Per sequence quality FastQ format

Vahid