SPAdes 3.10.0 assembly help

Question

[kaobrien2301]

Hi everyone,

I've been utilizing older versions of SPAdes to assemble a cohort of genomes, and recently switched to the most updated version to obtain more efficient assemblies. However, a few of my strains fail to assemble when I call SPAdes 3.10.0. These strains are not of the highest quality (when assembled in 3.05.0, many contigs are low coverage and below 100 bp), but can still be assembled in other software versions without much of the genome being discarded. Has any one else encountered this issue or have any insights as to why 3.10.0 would fail? Would it be best to try these strains using a different assembly method geared towards even shorter-read sequences?

Thanks!