Mismatch Melting Temps
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Entering edit mode
7.0 years ago

Anyone know where I can find a table for nearest neighbor melting temperatures with mismatched primers included. So something like http://www.pnas.org/content/95/4/1460/T1.expansion.html but with AT/TG, AT/TC, AA/GG... etc
Do not want a link to programs that will calculate it for me.

sequencing • 1.2k views
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