I have a large list of EC numbers and would like to do a functional analysis of what they are actually doing in the cell.
For that, which would be the best/most direct method to convert each EC to a COG functional category?
I have results for KEGG pathways, but a large part of my ecs (30%) can't be mapped to any pathway, besides I get 85 different categories that I can't group myself into broader ones. Kegg modules does even worse, 83 categories, 66% of ecs not in any module. Uniprot Pathway is also bad (can't annotate 57% of the ecs, although only 48 categories).
The worse from this is that the results of KEGG/Uniprot don't match at all (the categories are very different)
I would like to try COG, as the number of functional categories is smaller, and maybe I could cover more EC numbers.
Is biochemistry this diverse and unknown?? Anyone with more ideas to annotate EC numbers to biological processes/broad functions? (e.g. aminoacid metabolism, cofactor metabolism... etc)