What classifying or clustering tools should I apply for an exhaustive gene expression analysis?
0
1
Entering edit mode
3.9 years ago
Pin.Bioinf ▴ 290

Hello, I am working on a project and I have already done a differential expression analysis (a simple pairwise comparison with DESeq2).

I have 5 conditions:

  • infected with bacteria
  • infected with fly
  • not infected but exposed to bacteria
  • not infected but exposed to fly
  • untreated (NAIVE)

For each condition, I have 3 biological samples.

Samples were taken at 2, 7, 14 and 21 days post infection. (I figure I could do a Venn diagram with the genes that are the same in more than one condition, or maybe see the evolution of a gene depending on the time...)

I have many significant genes for each condition, and I would like to know what else I should do (Clustering those genes, or doing a temporal analysis, classification, PCA...) And if there are any examples online, tutorials or articles I could read to start practicing and to start applying to my experiment.

Thank you!

RNA-Seq R gene • 761 views
ADD COMMENT

Login before adding your answer.

Traffic: 1630 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6