Question: Demultiplexing fastq without barcode; with QIIME
gravatar for nidhiv
2.7 years ago by
nidhiv0 wrote:

I have received a single forward and reverse fastq file for 21 separate sample data generated by Illumina Miseq. The sequence appears to have the barcodes removed.

@M04771:133:000000000-B59PL:1:1101:16445:1361 1:N:0:0

Any suggestions as to how I go about separating it to each sample? Preferably with QIIME


sequencing • 1.2k views
ADD COMMENTlink modified 2.7 years ago by Brian Bushnell17k • written 2.7 years ago by nidhiv0

What Brian Bushnell says. You are missing the separate barcode FASTQ file which is required for demultiplexing (the -b option for

ADD REPLYlink modified 2.7 years ago • written 2.7 years ago by Chris Fields2.1k
gravatar for Brian Bushnell
2.7 years ago by
Walnut Creek, USA
Brian Bushnell17k wrote:

You need to obtain the original data; it is impossible with what you have (at least, with the information given). In special cases (such as when each is a distinct, unrelated organism, for which you have a complete reference) there are techniques you can use to split the file, but you should still get the original data.

ADD COMMENTlink written 2.7 years ago by Brian Bushnell17k
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