Crash Galaxy mapping of rna-seq on transcriptome
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6.8 years ago
profjdyme • 0

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Hello: in Galaxy anybody has had an experience like this? We're trying to map RNA-seq reads to a certified transcriptome from Vectorbase (bugs) but it crashed...

Tool Parameters Input Parameter Value Note for rerun Will you select a reference genome from your history or use a built-in index? history Use the following dataset as the reference sequence 6: Anopheles-albimanus-STECLA_TRANSCRIPTS_AalbS2.3.fa
Algorithm for constructing the BWT index Auto. Let BWA decide the best algorithm to use
Single or Paired-end reads paired
Select first set of reads 4: FASTQ Groomer on data 1
Select second set of reads 4: FASTQ Groomer on data 1
Enter mean, standard deviation, max, and min for insert lengths. Empty.
Set read groups information? do_not_set
Select analysis mode illumina
Job Resource Parameters no

RNA-Seq alignment Transcriptome Galaxy rna-seq • 1.2k views
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