Question: Adaptors Sequences and Trimming
0
gravatar for lzass18
2.6 years ago by
lzass1810
lzass1810 wrote:

Hi everyone,

I received a lot of good help last time, and I hope someone will be able to help me this time as well. I've been working on an ngs pipeline exercise, and I've managed to make good progress, but I still have many questions regarding adaptor sequences and trimming. Keep in mind, I'm only a beginner at this.

1) Given a certain sequencing platform, how does one determine which adaptors were used to generate the reads? Is this information provided by the service provider? And if not provided, how does one determine which adaptors to search for?

2) If an adaptor is fragmented, how do you search/trim a certain percentage of it?

3) Do the adaptors differ between targeted sequencing, whole exome sequencing and whole genome sequencing

4) Is there software/tool that can automatically ID adaptors and fragments of adaptors within sequencing reads?

Thank you in advance!

sequencing next-gen • 634 views
ADD COMMENTlink written 2.6 years ago by lzass1810
1

Are these homework questions? They look like!

ADD REPLYlink written 2.6 years ago by Macspider3.0k

If you received lots of good help last time, perhaps you should up vote the responses and comments you got and accept the answers that worked for you.

ADD REPLYlink written 2.6 years ago by Joseph Hughes2.8k
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