Entering edit mode
6.9 years ago
agata88
▴
870
Hi all!
I was testing different references (gg_13_8, gg_13_5) for the occurrence of several species and I saw that most of them are missed . I would like to add those sequences manually. The main problem is how to add it to make it compatible with qiime?
PS. How greengenes database is made?
Thanks in advance!
Best, Agata
Greengenes has not been updated in a long time. I suggest you try Silva.
Silva does not have those species too.
What are these species?
for example: Bacteroides cellulosilyticus DSM 14838
I ran this:
and got many entries. Among them:
So, it looks like it is present.
hmm, yes indeed. I was using Silva124 version not the one you have used. I am gonna go with 128 version. Thanks.