This is cross-posted with Increasing kmer limit in SPAdes, but because I may never receive a reply from that post, which is > 1 year old, I am re-posting afresh.
Based on assembly stats comparison at choice of k-mer size for metagenomic assembly, between k-mer=20 Vs higher values, I seek answers to these questions:
1.. Is increase of k-mer > 127 possible with SPAdes?
2.. Is it NECESSARY to check k-mer values > 127 for SPAdes? [Read length trimming, prior to SPAdes assembly, yielded trimmed length distribution of - 41- 150bp]
I've got 2*150bp (PE data) for fungal spores (haploid genome) where I want to play with higher k-mer values to assess any improvement in assembly contiguity and completeness. So sharing your experience would help me. Thank you!