Mothur_already merged miseq_sequences_how can I use make.contigs?
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6.7 years ago
yusookzzz • 0

Hi! Ìm using mothur when I have to qualify the paired end miseq raw sequences

Usually I made stability files and undergo make.contigs(files=stability.files) but

Recently I got data that already merged sequences (miseq paired end)

As you already know, input for a make contigs command, should be a stability.files that made of forward and reverse fastq

What should I do ??? with already merged fastq files ???

If there is no solution,,, I have to request raw data before merge but It should be a final way...

Somebody help..

sequencing next-gen software error • 1.2k views
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Entering edit mode
6.7 years ago
yusookzzz • 0

oh and I already use fastq.info() command

but it is not for a miseq paired end uses ...

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