Question: Tools for investigating the correlation of differential gene expression & histone marks/ TF binding?
1
gravatar for chiefcat
2.1 years ago by
chiefcat110
chiefcat110 wrote:

Hi everyone.

Would you please share some tools which can integrate differential gene expression & differential ChIP enrichment data?

I have both types of data from 2 time points (Stem cells vs differentiated cells) and I would like to investigate if the change of histone mark enrichment correlates with the differential gene expression in these two cell types.

Many thanks!!!

rna-seq integration chip-seq • 879 views
ADD COMMENTlink modified 2.1 years ago by amy.bashir80 • written 2.1 years ago by chiefcat110
0
gravatar for shoujun.gu
2.1 years ago by
shoujun.gu370
Rockville/MD
shoujun.gu370 wrote:

may be you can try bedtools intersect to get the overlapped region between RNASeq result (eg. -1000bp upstream) and ChIPSeq result.

ADD COMMENTlink written 2.1 years ago by shoujun.gu370

Hi Shoujun. Thanks, I am looking into the tutorial on their website now. May need to ask you some more questions later! =] Thanks!

ADD REPLYlink written 2.1 years ago by chiefcat110

Hi Shoujun, I've just got the Cuffdiff output found the labmate who help me to handle the RNA-seq. data, what kind of corrdinate I should use for the bedtools intersect? Do you have any examples in your hand? Thanks!

ADD REPLYlink written 2.1 years ago by chiefcat110

The easy way is to use ChIPSeq annotation tools (like PAVIS) to get the gene list and then compare with your RNASeq DEG list. Or to expand the region of your RNASeq DEG list with bedtools slop, and intersect with your ChIPSeq peak.

for bedtools slop usage and detailed coordinate, it's really depends on your specific goal. You should check http://bedtools.readthedocs.io/en/latest/content/tools/slop.html

ADD REPLYlink written 2.1 years ago by shoujun.gu370

Thanks Shoujun, what kind of tools can be used for the visualization of the intersection result? Do you just usually present it using Venn diagram other any other better way which can really show the changes of all genes between two samples?

ADD REPLYlink written 2.1 years ago by chiefcat110

usually I just use very standard visualization methods, like heatmaps for DEG and igv for peak examples. Maybe there is some fancy tools, but I'm not familiar with that.

ADD REPLYlink written 2.1 years ago by shoujun.gu370
0
gravatar for amy.bashir
2.1 years ago by
amy.bashir80
amy.bashir80 wrote:

I think BETA (http://cistrome.org/BETA/) is well suited for this task.

ADD COMMENTlink written 2.1 years ago by amy.bashir80

Hi Amy, thanks for your reply. I've read a little bit about BETA but the example on their website is too simple. In the test example, only one bed file is input for the analysis. In my case, should I input the differential enriched peaks between the two time point of samples? Thanks.

ADD REPLYlink written 2.1 years ago by chiefcat110
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