Question: is it possible to do alignment using only the reverse strand as single end ?
2.3 years ago by
cjgunase • 30
cjgunase • 30 wrote:
I tried to do alignment using only the reverse strand FASTQ file and the mapping efficiency was very low(0.7%). When using both strands 85% (as paired-end) and using forward strand again 87% (as single -end). What is the reason for this?
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