Question: is it possible to do alignment using only the reverse strand as single end ?
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gravatar for cjgunase
2.3 years ago by
cjgunase30
United States
cjgunase30 wrote:

Hello,

I tried to do alignment using only the reverse strand FASTQ file and the mapping efficiency was very low(0.7%). When using both strands 85% (as paired-end) and using forward strand again 87% (as single -end). What is the reason for this?

bismark alignment dna-seq • 667 views
ADD COMMENTlink written 2.3 years ago by cjgunase30
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