Question: Predicted complete ORFs that are identical to cDNA clones but have no BLAST hits
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gravatar for nancydong20
2.6 years ago by
nancydong20100
nancydong20100 wrote:

Hello!

I assembled a number of transcripts from a non-model organism and used TransDecoder to predict ones with complete ORFs. I see that a number of the transcripts containing complete ORFs don't have a hit in protein or RNA BLAST databases, but are identical to entries in the BLAST EST database from the same species. What are these transcripts? Are they protein-coding genes that don't have homology with known genes, or are they pseudogenes? Or both?

Thank you very much!

rna-seq • 628 views
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