I have some paired-end RNA-seq data from a collaborator. I was told that they are "stranded", but nothing about whether it's RF or FR.
I pulled out 5 pairs of reads (read 1 and read 2) from each of the four RNA-seq libraries and aligned them to the reference genome of the organism. I see that 50% of the read 1s map to the "+" strand, the other 50% to the "-" strand. Same as the read 2s.
Does this mean that the data is actually unstranded?
Any help would be greatly appreciated!