Question: PCT_ribosomal base and PCT_intergenic base in standard RNA-Seq
gravatar for CY
3.1 years ago by
United States
CY550 wrote:

I have been using STAR for RNA-Seq alignment.

For alignment QC based on the bam file, Picard indicates a wild range of percentage of ribosomal base (10%-50%) and percentage of intergenic base (10%-50%) across samples. I am not sure if this is normal

I assume the percentage of ribosomal base depends on the RNA selection method and percentage of intergenic base should be relatively low, right?

Can anyone share some insights on this? Thanks

rna-seq qc • 580 views
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