Question: Gene level from transcript level expression estimate.
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mforde84 • 1.3k wrote:
Would it be appropriate to simply sum FPKM/RPKM/TPM normalized data across transcripts to generate a gene level estimate? Each transcript will be abundant at a certain % of total expression, so the intergration should simply be the gene level expression of all associated transcripts. Am I correct or am I missing something?
I'd think the answer is no, depending on how 'gene level estimate' defined. Since transcripts associated with a gene can share exons, I'd think summing counts including overlapping transcripts from the same gene would lead to multiple counting. Or did you mean summing reads over all exons associated with a gene locus?