If I understand right, MACS shifts chip-seq tags of a fragment into 3´end because the tags are just small sequences in the extremes of the fragment. But why does it only apply to the ChIP sample and not to the control samples (input DNA)? Or paired-end sequencing isn't done in control samples?
Question: ChIP-seq: Why MACS doesn't apply tag shifting to the control sample?
14 days ago by
salamandra • 70
salamandra • 70 wrote:
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