Hi all I am using maSigPro package analying my time-series data. And my time include 0 time. Please check if it is right for my design. There is only 300 DEGs(PHvsControl) with no enriched GO terms. The arguments I selected are as follows degree=5, vars="group",rsq=0.6 Thanks a lot. Time Replicate Control PH LR0h1 0 1 1 0 LR0h2 0 1 1 0 LR0h3 0 1 1 0 LR2h1 2 2 0 1 LR2h2 2 2 0 1 LR2h3 2 2 0 1 LR6h1 6 3 0 1 LR6h2 6 3 0 1 LR6h3 6 3 0 1 LR12h1 12 4 0 1 LR12h2 12 4 0 1 LR12h3 12 4 0 1 LR24h1 24 5 0 1 LR24h2 24 5 0 1 LR24h3 24 5 0 1 LR30h1 30 6 0 1 LR30h2 30 6 0 1 LR30h3 30 6 0 1 LR36h1 36 7 0 1 LR36h2 36 7 0 1 LR36h3 36 7 0 1 LR72h1 72 8 0 1 LR72h2 72 8 0 1 LR72h3 72 8 0 1 LR120h1 120 9 0 1 LR120h2 120 9 0 1 LR120h3 120 9 0 1 LR168h1 168 10 0 1 LR168h2 168 10 0 1 LR168h3 168 10 0 1