Question: WES chromosome Y off-target reads coverage compare to autosome
gravatar for hujingchu
17 months ago by
hujingchu0 wrote:

Hi all, I have couple of WES samples using different capture kits, and would like to predict their gender by evaluating the off-target reads from chromosome Y and autosome(like chr20), some thing strange is the off-target reads per 1M of chrY is always only 10% of autosome(chr20 here), which is far away from my expectation (50%) in Male. Is there any explanation why this happend ?

sequencing coverage alignment • 532 views
ADD COMMENTlink modified 17 months ago by markus.riester470 • written 17 months ago by hujingchu0
gravatar for markus.riester
17 months ago by
markus.riester470 wrote:

Should be much lower because of the very low mappability of chrY. If you have WES, then why using off-target reads? For most kits, you will have plenty of perfectly mapping exons that will show 0 reads in females (or very few in case of minor cross-sample contamination).

ADD COMMENTlink written 17 months ago by markus.riester470
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