Question: QUAST Icarus Coverage across reference genome with pacbio data
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gravatar for matthewrgemmell
5 months ago by
University of Liverpool
matthewrgemmell0 wrote:

I have been trying to use QUAST to produce icarus output with the coverage across the reference assembly. I am trying to do this with PacBio data and I am having issues getting it to work. I aligned my pacbio reads to the reference using pbalign and input the resulting bam file into the quast command with the --bam option.

It does produce a icarus image but without the coverage info. I think it may not be working as it is not paired data but I am not certain. Has anybody else had the same issue? Does anyone know a fix/how to do it correctly?

Thanks for reading

Matthew gemmell

alignment assembly genome • 234 views
ADD COMMENTlink written 5 months ago by matthewrgemmell0
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