High CG>CA and CG>TG errors from bait bias WES
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3.8 years ago
maduh17 ▴ 10

Hi there,

I've ran picard's CollectSequencingArtifactMetrics on my WES samples, both on my fresh-frozen tumors and blood normals.

In trinucleotide context, I'm seeing high "bait bias" errors CG>CA and CG>TG in both tumors and normals. These two error types cluster separately from other trinucleotide errors.

Is this something I should be alarmed about?

OxoG, FFPE metrics, Cref and Gref metrics are fine.

This is my first time working with WES data in general.

Many thanks.

WES bias • 742 views
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