Question: High CG>CA and CG>TG errors from bait bias WES
0
gravatar for maduh17
14 months ago by
maduh1710
Kuala Lumpur, Malaysia
maduh1710 wrote:

Hi there,

I've ran picard's CollectSequencingArtifactMetrics on my WES samples, both on my fresh-frozen tumors and blood normals.

In trinucleotide context, I'm seeing high "bait bias" errors CG>CA and CG>TG in both tumors and normals. These two error types cluster separately from other trinucleotide errors.

Is this something I should be alarmed about?

OxoG, FFPE metrics, Cref and Gref metrics are fine.

This is my first time working with WES data in general.

Many thanks.

bias wes • 299 views
ADD COMMENTlink written 14 months ago by maduh1710
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1343 users visited in the last hour