Question: using MACS2 with hg38
0
gravatar for varsha619
3 months ago by
varsha61970
varsha61970 wrote:

Hi! I am wondering if there has been any updates regarding using MACS2 with hg38. I have my files aligned to hg38 and used genome option in MACS2 as default for 'hs' which I am not sure is still hg18 or if it has been updated to hg38. Please let me know, thank you!

hg38 macs2 • 206 views
ADD COMMENTlink modified 3 months ago by igor6.2k • written 3 months ago by varsha61970
0
gravatar for igor
3 months ago by
igor6.2k
United States
igor6.2k wrote:

The -g/--gsize parameter in MACS is "the mappable genome size or effective genome size which is defined as the genome size which can be sequenced". It's not really the actual reference sequence. The mappable genome size has not changed much (especially as it applies to short reads). Even if you assume it changed significantly, there are still big differences between different variants of hg38.

ADD COMMENTlink written 3 months ago by igor6.2k

Hi Igor, thank you for your reply. Do you think it's ok for the files to be aligned to hg38 and MACS2 run with the -g/--gsize parameter?

ADD REPLYlink written 3 months ago by varsha61970
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1839 users visited in the last hour