Question: How to validate results of gene expression clustering in additional datasets?
gravatar for octopuslegs11
2.8 years ago by
octopuslegs110 wrote:

Hi all,

I am new to RNA-seq analysis and so am a little bit lost. The RNA-seq dataset I am working with is a time-course experiment with replicates. I have performed k-means clustering on a subset of genes and have identified 6 unique expression patterns of genes that co-vary throughout time. Bootstrapping of these clusters suggests that they are robust. I would like to now validate these gene clusters in a second data set (microarray data, also a time-course, albeit slightly different time-points so I am not expecting it to be perfect). How can I go about this?

Thanks in advance.

rna-seq • 785 views
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