Question: Identifying bacterial resistance using plasmid sequencing
gravatar for mohamed_refaat.1992
2.4 years ago by
mohamed_refaat.19920 wrote:

Hi everyone,

I'm working on a project studying plasmid-borne bacterial resistance in clinical settings. After culturing, plasmids from different samples were isolated and paired-end sequenced using Miseq.

I proposed the following sample-wise pipeline for identification of anti-microbial genes, plasmids and resisted drugs :

2 separate fastq files per sample ===> de-novo assembly using plasmidSpades on PATRIC online service ===> upload contig files to the following web-based tools:

  1. kmerFinder & PlasmidFinder for plasmids identification.
  2. kmerResistance for antimicrobial genes identification.

Can someone validate this workflow? will it eventually help me identify anti-microbial genes and plasmids present in each sample? How can I improve/optimise it ?

I've just joined the lab and this is the first project I get involved in. Any suggestions or notes will be highly appreciated.

Thanks in advance.

resistance plasmid • 523 views
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