Select differential expressed genes by SCDE package
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6.0 years ago
szhaoaf ▴ 40

Hey! Everyone,

I am doing single cell RNA seq analysis recently and classified my total 76 single cell samples into 3 groups by unsupervised hierarchy clustering analysis. My pseudo-time analysis showed it was pretty good to observe the time-line differentiation process.

Then I did differential expression genes analysis by SCDE package. However, I am confused with selecting differential expressed genes since the output results include 'z' value instead of 'p value' plus 'LogFold change'.

In this case, do I need to, in some way?, transfer the z value to p value? Is z value is the only standard for selecting genes?

I am glad to talk you with more about scRNA-seq!

Bests Shizheng

rna-seq • 1.2k views
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