Question: Use normalised reads for HISAT2/Braker1
gravatar for ersgupta
2.2 years ago by
ersgupta0 wrote:

I want to use braker for gene prediction of a denovo genome assembly using RNAseq data. I have 21 RNAseq samples.

My question is, for aligning using HISAT2, is it ok to use the normalised reads (using trinity: coverage 50) or use all 21 RNAseq files separately?

Any suggestion would be appreciated. Thanks

ADD COMMENTlink modified 2.2 years ago • written 2.2 years ago by ersgupta0
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