Create SINTAX reference database for metabarcoding
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4.2 years ago

Hi everyone,

I have been searching for a way to create a reference database that I would like to ultimately use with USEARCH to assign taxonomy to OTU's, using the sintax command. I am struggling to reformat my FASTA files to have SINTAX-compatible taxonomy annotations.

I just wanted to know if anyone has attempted this before or has any suggestions to create this type of database?

More details: I have compiled a fasta file from sequences (plants, trnL) downloaded from GenBank.

database USEARCH UPARSE metabarcode SINTAX • 1.1k views

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