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                    7.6 years ago
        ZheFrench
        
    
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    590
    eRNAs are bi-directionally transcribed. I was wondering how to separe them from a bam to make distinct bigwig using orientation and strand appartenance.
Do you want to identify bidirectional transcripts ? or want to create a bigwig that displays transcripts in a strand specific manner ?
Identify bidirectional transcripts, separe them into two different bam, to make 2 bigwigs to display distinctly depending both orientation.