Hi! I'm new and currently doing masters in bioinformatics. My DNA data is sequenced using PacBio sequencing. I used MECAT to assemble my DNA data which the output consists of 800 contigs, 51% GC content, 54Mbp (validation by using QUAST). What is the next step? Do I need to combine my contigs into scaffold? Is there any software (for annotation) for PacBio analysis besides software provided by PacBio? Thanks!
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