Entering edit mode
5.5 years ago
RemiToAmigo
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0
Hi all,
I’m writing to ask your opinion. I’ve been ask to perform an miRNA DE analysis….so far so good. miRNA from tissue A vs miRNA from tissue B.
There is such a great difference between the concentration of miRNA in A vs B that I’m worried that if I do a straight DE analysis, my results will be biased. Some of the A samples have been sequenced up ~30M reads and barely have 100k miRNAs mapped where some of the Bs have ~5M reads and have 1M miRNAs mapped….
Do you know any way to factor this in? I was thinking of instead doing a miRNA DE analysis, I would do a smRNA analysis...then the proportions would be factored in because the A samples have a lot of smRNAs compared to B samples ...any thoughts?
Cheers!
