Question: miRNA DE analysis from different tissues with different concentrations
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gravatar for RemiToAmigo
6 months ago by
RemiToAmigo0 wrote:

Hi all,

I’m writing to ask your opinion. I’ve been ask to perform an miRNA DE analysis….so far so good. miRNA from tissue A vs miRNA from tissue B.

There is such a great difference between the concentration of miRNA in A vs B that I’m worried that if I do a straight DE analysis, my results will be biased. Some of the A samples have been sequenced up ~30M reads and barely have 100k miRNAs mapped where some of the Bs have ~5M reads and have 1M miRNAs mapped….

Do you know any way to factor this in? I was thinking of instead doing a miRNA DE analysis, I would do a smRNA analysis...then the proportions would be factored in because the A samples have a lot of smRNAs compared to B samples ...any thoughts?

Cheers!

rna-seq • 120 views
ADD COMMENTlink written 6 months ago by RemiToAmigo0
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