Question: CAGEFightR to get CTSS from CAGE error
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gravatar for bharata1803
6 months ago by
bharata1803420
Japan
bharata1803420 wrote:

Hello,

So, I got bam files from CAGEseq experiment from FANTOM5. I realigned it to Ensembl GRCh 38 90 using bwa and then extract the bigwig file using deeptools bamCoverage.

Then I am trying to get the CTSS using CAGEFightR from the bigwig files (plus and minus strand) I have generated.

Using the quantifyCTSSs from CAGEFightR function, I got error "Error in asMethod(object): all the ranges in the object to coerce to GPos must have a width of 1"

What is wrong with my method?

cage-seq bioconductor • 174 views
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