Question: Correct Interval Coverage data for overall number of raw reads
0
gravatar for AP
16 months ago by
AP90
AP90 wrote:

Hello,

I have two datasets with Interval Coverage metrics that I would like to compare. However, both datasets have a significant different amount of raw reads. I would like to normalise or correct for this different amount of raw reads in order to make the interval data coverage comparable.

Any suggestions? I thought of correcting for an effect of raw reads or standardized all of the interval coverage data.

Any help would be greatly appreciated.

Thank you!

sequencing • 273 views
ADD COMMENTlink modified 16 months ago • written 16 months ago by AP90
0
gravatar for AP
16 months ago by
AP90
AP90 wrote:

Two ways of doing it:

  1. Downsizing: can be done using samb: http://lomereiter.github.io/sambamba/
  2. Normalise the data to a mean of zero and sd of 1

I guess I answered my own question... :)

ADD COMMENTlink written 16 months ago by AP90
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