Question: Statistical comparison of expression of two _different_ genes
1
gravatar for kristoffer.vittingseerup
6 months ago by
European Union
kristoffer.vittingseerup1.8k wrote:

I am interested in a comparing expression of gene_A vs gene_B in a homogenous population. If you don't have the number of samples to do a non-parametric test how would one do a parametric statistical comparison?

My thoughts are as follows:

  1. Gene length normalisation is essential. TPM (transcript per million) or equivalent.
  2. Inter-library normalization needed
  3. Log transormation needed (to make it approx norm distributed)
  4. Should probably be a paired test
  5. Variance is most likely different in the different genes

Can one do a paired Welch test?

Do you have other ideas for how to do this?

In advance - thanks!

ADD COMMENTlink written 6 months ago by kristoffer.vittingseerup1.8k

I think you could probably use log2(TPMs) and then a paired Welch test.

ADD REPLYlink modified 6 months ago • written 6 months ago by chris86290

Does such a thing exists?

ADD REPLYlink written 6 months ago by kristoffer.vittingseerup1.8k

It seems not. Your probably going to have to do a non-parametric test.

ADD REPLYlink modified 4 months ago • written 6 months ago by chris86290
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