Question: (Closed) Fragment size for Illumina Miseq 2 x 250-bp sequencing
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gravatar for kat
19 months ago by
kat10
kat10 wrote:

I am simulating Illumina MiSeq 2 x 250-bp data with ART (https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) and am wondering the optimal fragment size (mean and st. dev.) for sequencing on this platform. Is there a recommended fragment size or insert size for shearing DNA prior to sequencing? Thank you!

ADD COMMENTlink written 19 months ago by kat10

Hello kwalter!

We believe that this post does not fit the main topic of this site.

This is a wet-lab rather than a bioinformatics question. Consider posting on StackExchange or SeqAnswers, but as this is a standard Illumina question, browse their documents first.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 19 months ago • written 19 months ago by ATpoint36k
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