Question: (Closed) A question about drop-seq and Nextera XT Library prepare
gravatar for 610225668
21 months ago by
6102256680 wrote:

Hi guys!

Recently,I was reading a paper about drop-seq and see the serve provided by illunima( It said they use the Nextera XT Library Preparation Kit for the cDNA. In my impression , Nextera XT Library Preparation Kit using Tn5 to interrupt the DNA meanwhile adding adapter for sequencing .But the cDNA in drop-seq was made up of PCR primer + cell barcode +UMI +cDNA. I am confused if the cDNA was interrupted, the fragment may not contain cell barcode and UMI,and the position which represent the cell barcode and UMI will be indeterminate.

dropseq rna-seq cdna • 952 views
ADD COMMENTlink written 21 months ago by 6102256680

Hello 610225668!

We believe that this post does not fit the main topic of this site.

Not a bioinformatics question.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink written 21 months ago by lakhujanivijay5.2k

Please post this over at

ADD REPLYlink written 21 months ago by genomax89k
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