Question: (Closed) A question about drop-seq and Nextera XT Library prepare
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gravatar for 610225668
10 months ago by
6102256680
6102256680 wrote:

Hi guys!

Recently,I was reading a paper about drop-seq and see the serve provided by illunima(https://www.illumina.com/science/sequencing-method-explorer/kits-and-arrays/drop-seq.html) It said they use the Nextera XT Library Preparation Kit for the cDNA. In my impression , Nextera XT Library Preparation Kit using Tn5 to interrupt the DNA meanwhile adding adapter for sequencing .But the cDNA in drop-seq was made up of PCR primer + cell barcode +UMI +cDNA. I am confused if the cDNA was interrupted, the fragment may not contain cell barcode and UMI,and the position which represent the cell barcode and UMI will be indeterminate.

dropseq rna-seq cdna • 633 views
ADD COMMENTlink written 10 months ago by 6102256680

Hello 610225668!

We believe that this post does not fit the main topic of this site.

Not a bioinformatics question.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 10 months ago by lakhujanivijay4.5k

Please post this over at SeqAnswers.com.

ADD REPLYlink written 10 months ago by genomax73k
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