I have a project in which I want to compare the absolute abundance of TCR across patients.
I have no problem deriving relative abundance of each TCR using TCR-Seq. How about absulote abundance?
With DNA barcode, we can calculate the amount of original fragment before PCR. Beside, I can use the same initial blood volumn. However, how can we control the initial DNA input during Library prep (Appearently, we can't use the same amount of DNA input to start because that would be pointless). Is there anyway we can do this without adding spike-in?