Question: help with interpreting metagenomic metatranscriptomic data
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gravatar for bitpir
6 days ago by
bitpir100
bitpir100 wrote:

Hi, I'm not quite certain how to interpret the data below and would like to seek your opinion on it. I have meta-genomic and meta-transcriptomics of a sample, and calculated the relative abundance/activity of each taxa. The rel. activity/abundance are calculated as proportion of reads mapped to each taxa. When I plotted RNA vs DNA, the spearman correlations are pretty low ~0.3. However, when I plotted the RNA/DNA ratio vs DNA rel. abundance, there is negative correlation between the 2. Is it correct then to interpret that more abundant taxa tend to have lower RNA/DNA ratio, hence less active? Or am I simply plotting1/DNA vs DNA, which obviously will give me a negative correlation?

Thanks so much for the help!

Left:Graph of RNA TPM vs DNA relative activity. Right: Graph of RNA(TPM)/DNA(rel. abundance) ratio vs DNA relative abundance

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