Entering edit mode
5.2 years ago
lamia_203
▴
100
Im trying to run normalization using SCnorm in R. The normalization is running fine on the first dataset but the second dataset is coming up with an error message:
Error in if (any(colSums(SingleCellExperiment::counts(Data)) == 0)) { :
missing value where TRUE/FALSE needed
When checking the matrix by eye there were no missing values but when checking using is.na function all samples/columns came up as containing missing values. Both datasets were given the same parameter when ran in Linux using STAR and imported the same way so I don't understand why this error message is appearing in the second dataset.
