What is Feature Barcoding technology?

Question

[phamtrongthang123]

I heard the term Feature Barcoding a lot so I find resources to understand it. I have read docs of 10xgenomics and I see that Feature Barcoding technology is a method for adding extra channels of information to cells by running single-cell gene expression in parallel with other assays, this technology also do something with t-SNE and something with matrices.

I can't imagine the whole picture because I don't know what are those adding extra channels of information to cells, single-cell gene expression and parallel with other assays mean. And what is the input and output of this technology? Is there any paper go along with this technology?

Answer 1

[Jean-Karim Heriche]

As far as I can tell Feature Barcoding is the name given by 10x Genomics to the CITE-seq method or some variant of it. It consists in using oligonucleotide barcodes on antibodies targeting cell surface proteins.

EDIT: Checking 10X Genomics page, they use feature to mean any barcoded reagent, e.g. antibody or CRISPR guide RNA.

[phamtrongthang123]

Thanks a lot. I'm really confused at first because can't find any exact match of the term Feature Barcoding on some papers that 10x genomics mentioned. Now it's much clear to me. Thanks again.