Question: Problem in Trinity Assembly. How can I fix it?
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gravatar for trinimaclean
3 months ago by
trinimaclean0 wrote:

Hi! I am trying to assembly transcripts by using Trinity Assembly. I am running the following command:

Trinity --seqType fq --left 121A.200.left.fastq --right 121A.200.right.fastq --CPU 8 --max_memory 20G --min_contig_length 150 --verbose

It starts to run ok, but then we got this problem:

Error encountered::

Error, cmd: mv /home/student2/project_chili/trinity_out_dir/read_partitions/Fb_0/CBin_97/c9751.trinity.reads.fa.out/inchworm.K25.L25.DS.fa.tmp /home/student2/project_chili/trinity_out_dir/read_partitions/Fb_0/CBin_97/c9751.trinity.reads.fa.out/inchworm.K25.L25.DS.fa 2>tmp.3323.1551885108.stderr died with ret 256 at /home/student2/trinityrnaseq-Trinity-v2.8.4/PerlLib/Pipeliner.pm line 186. Pipeliner::run(Pipeliner=HASH(0x558a39c1eec8)) called at /home/student2/trinityrnaseq-Trinity-v2.8.4/util/support_scripts/../../Trinity line 2517 eval {...} called at /home/student2/trinityrnaseq-Trinity-v2.8.4/util/support_scripts/../../Trinity line 2512 main::run_inchworm("/home/student2/project_chili/trinity_out_dir/read_partitions/"..., "/home/student2/project_chili/trinity_out_dir/read_partitions/"..., undef, "") called at /home/student2/trinityrnaseq-Trinity-v2.8.4/util/support_scripts/../../Trinity line 1650 main::run_Trinity() called at /home/student2/trinityrnaseq-Trinity-v2.8.4/util/support_scripts/../../Trinity line 1345 eval {...} called at /home/student2/trinityrnaseq-Trinity-v2.8.4/util/support_scripts/../../Trinity line 1344

Do you know what it means and how can we fix it?

Thank you very much in advance!

trinity assembly • 159 views
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