Question: How to analyse a list of DEG (differentially expressed genes) and DASG (differentially alternatively splices genes)
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gravatar for darbinator
9 weeks ago by
darbinator90
darbinator90 wrote:

Hello,

I'm studying a protein on A.thaliana that is suspected to have a role on the transcription and on the alternatively splicing.

I have RNA-Seq data with 12 samples:

Control (x3)

Mutants (x3)

Control (heat-shock) (x3)

Mutants (heat-shock) (x3)

I perform DEG analysis (DESeq2) and DASG analysis (rMATS) with this design: Controls vs Mutants

and Controls (heat shock) vs Mutants (heat-shock)

And I then I grouped the DEG (110 genes) and DASG (30 genes) which were found in common in both conditions.

But now I'm a little bit lost about analysis this results. Gotonology does not generate a particular profile (because there is few genes)..

How can I analyze this list of genes ? By analyzing gene individually ?

Thank's by advance

deg rna-seq analysis ngs • 125 views
ADD COMMENTlink written 9 weeks ago by darbinator90
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