Question: How to analyse a list of DEG (differentially expressed genes) and DASG (differentially alternatively splices genes)
gravatar for darbinator
22 months ago by
darbinator230 wrote:


I'm studying a protein on A.thaliana that is suspected to have a role on the transcription and on the alternatively splicing.

I have RNA-Seq data with 12 samples:

Control (x3)

Mutants (x3)

Control (heat-shock) (x3)

Mutants (heat-shock) (x3)

I perform DEG analysis (DESeq2) and DASG analysis (rMATS) with this design: Controls vs Mutants

and Controls (heat shock) vs Mutants (heat-shock)

And I then I grouped the DEG (110 genes) and DASG (30 genes) which were found in common in both conditions.

But now I'm a little bit lost about analysis this results. Gotonology does not generate a particular profile (because there is few genes)..

How can I analyze this list of genes ? By analyzing gene individually ?

Thank's by advance

deg rna-seq analysis ngs • 768 views
ADD COMMENTlink written 22 months ago by darbinator230
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 967 users visited in the last hour