I'm studying a protein on A.thaliana that is suspected to have a role on the transcription and on the alternatively splicing.
I have RNA-Seq data with 12 samples:
Control (heat-shock) (x3)
Mutants (heat-shock) (x3)
I perform DEG analysis (DESeq2) and DASG analysis (rMATS) with this design: Controls vs Mutants
and Controls (heat shock) vs Mutants (heat-shock)
And I then I grouped the DEG (110 genes) and DASG (30 genes) which were found in common in both conditions.
But now I'm a little bit lost about analysis this results. Gotonology does not generate a particular profile (because there is few genes)..
How can I analyze this list of genes ? By analyzing gene individually ?
Thank's by advance