Question: Determining expressed genes when average gene LogCPM distribution is unimodal
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12 months ago by
cfos4698140 wrote:

Hi all,

Determining which genes are expressed in an RNA-seq experiment is often a little arbitrary, but one method I've seen is to look at the average logCPM distribution of all genes in the data set (described here When the average logCPM distribution distribution is bimodal (the typical case), one would choose a threshold in the "trough" between the peaks.

I've tried this method, and found that the distribution is unimodal (image at the end of the post, with arbitrary cutoff line of -1). Does anyone have a suggestion for what to do in this case?


Average gene LogCPM distribution

rna-seq rnaseq • 360 views
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