Merge MNase-seq bam files from replicates.
0
3
Entering edit mode
5.1 years ago
kashiff007 ★ 1.9k

I am working with MNase-seq data. I have few replicates from samples. For the analysis, I have to work on distogram (histograms of distances between mapped reads’ start positions aligning in opposing orientation), phasogram (histograms of distances between mapped reads’ start positions aligning in the same orientation) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212987/ and few more parameters.

Example of distogram and phasogram

Question: Since all these analysis can be done on each bam files, is it OK to merge the bam files from replicates to get the mean value? If it is OK then how to merge these bam replicates?

next-gen MNase-seq bam-files merge replicates • 804 views
ADD COMMENT

Login before adding your answer.

Traffic: 1968 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6